False negatives can occur when a test is taken too early, when enough time has not lapsed to allow a sufficient amount of HCG to be produced. A false negative can also occur when first morning urine is not used. While it is not always required, first morning urine does contain the most HCG, and if a trace amount is present, first morning would be the most likely to produce a positive. If the test was used incorrectly, a false negative can also occur.

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